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Investigating the roles of eIF4F and eIFiso4F in Arabidopsis thaliana (566.7)
Author(s) -
Cole Nicola,
Browning Karen
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.566.7
Subject(s) - arabidopsis thaliana , biology , arabidopsis , eif4a , immunoprecipitation , messenger rna , gene isoform , translation (biology) , eukaryotic translation , microbiology and biotechnology , protein subunit , rna , ribosomal rna , initiation factor , translational regulation , eukaryotic initiation factor , genetics , gene , mutant
Eukaryotic initiation factor (eIF) 4F, the cap‐binding complex, binds to the messenger RNA (mRNA) in an early step in eukaryotic protein synthesis to recruit the mRNA to the protein making or translational machinery, the 40S ribosomal subunit. Higher plants such as Arabidopsis thaliana contain a plant‐specific cap‐binding complex, eIFiso4F, in addition to the conserved eIF4F complex found in eukaryotes. Genotypic and phenotypic differences between these complexes suggest functional differences. In an effort to define these functional differences, experiments performing RNA immunoprecipitation (RNA‐IP) using wild type Arabidopsis thaliana plant tissue, followed by deep sequencing, have been carried out to determine if there are different mRNA populations bound by the two different isoforms. Messages found to be differentially bound by one isoform over the other will be validated using other methods such as quantitative real time polymerase chain reaction (qRT‐PCR) and In vitro translation assays. Grant Funding Source : Supported by NSF