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rRNA processing intermediates may not contribute to ribosome biogenesis (560.9)
Author(s) -
Nazar Ross,
Van Loggerenberg Warren,
Nellimarla Srinivas
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.560.9
Subject(s) - ribosome biogenesis , ribosomal rna , ribosome , biogenesis , 5.8s ribosomal rna , biology , cleavage (geology) , microbiology and biotechnology , rna , computational biology , genetics , gene , paleontology , fracture (geology)
Much of the ribosomal RNA in a eukaryotic ribosome is derived from a common nucleolar transcript via a seemingly complex cleavage pathway which can vary greatly from organism to organism. In S, cerevisiae, for example, two steps have been reported for the five 5’ ETS but in S. pombe as many as six intermediates have been observed. The significance of these steps in for ribosome biogenesis remains unclear including the differences which are observed between organisms. In this study, the processing of the 5’ ETS in S. pombe cells has been reexamine using RT‐PCR, ligation‐mediated RT‐PCR and targeted mutagenesis. The results suggest that some intermediate steps are very minor or may be artifacts of the mapping techniques. More important, the complete removal of a cleavage site can leave levels of mature rRNA essentially unchanged. Taken together, such observations raise the possibility that at least some intermediates are unnecessary, are rare events or even experimental artifacts which do not contribute to rRNA maturation or ribosome biogenesis. Grant Funding Source : Supported by the Natural Sciences and Engineering Research Council of Canada