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Investigating the role of 53BP1 in DNA repair (554.1)
Author(s) -
Flores Desirey,
Grosesser Torsten,
Hatherill J. Robert
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.554.1
Subject(s) - micronucleus test , dna damage , radiosensitivity , ionizing radiation , microbiology and biotechnology , dna repair , cell culture , dna , carcinogen , biology , cancer research , chemistry , immunology , genetics , toxicity , irradiation , medicine , radiation therapy , physics , organic chemistry , nuclear physics
Hypothesis: 53BP1 foci will return to baseline 24 hrs after radiation exposure, but it will take the BALB/c longer due to their increased radiosensitivity. P53 binding protein No.1 (53BP1) has been shown to accumulate in nuclear areas of a cell that have extensive DNA damage such as double strand breaks (DSB). DSBs are the worse type of DNA damage and cells that have been exposed to damaging agents such as ionizing radiation are susceptible to developing cancer. Micronuclei (MN) were scored as markers of induced DNA damage, which appear as extra nuclear bodies that are formed from un‐ or mis‐rejoined DSBs. In the present studies cells from BALB/c and SPRET/EiJ mice were used to investigate the effects of acute X‐rays and prolonged 32P exposures. 53BP1 foci were imaged after acute exposures and MN were scored after prolonged exposures. BALB/c and SPRET/EiJ cells were challenged with an X‐ray dose of 1 Gy and immunostained to evaluate the number of foci. Both cell lines were also exposed to 32P over a prolonged amount of time to receive a total dose of 0.4 Gy and were then scored for MN. After the acute exposures the number of foci was higher in the cells derived from BALB/c mice compared to SPRET/EiJ cells and after 24 hrs the amount of foci returned to baseline in both cells lines. We conclude that cells from BALB/c mice exposed to radiation sustained more DNA damage than the SPRET/EiJ cell line and 53BP1 foci are localized to areas of DNA damage. Grant Funding Source : NSF ATE DUE 1205059