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Creatine influence on acute high and low ethanol exposure on cardiac heart muscle (547.5)
Author(s) -
Ezeamama Precious,
Haddad Gabriel
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.547.5
Subject(s) - creatine , creatine kinase , medicine , alcohol , cardiomyopathy , endocrinology , protein kinase b , pi3k/akt/mtor pathway , cardiac muscle , cardiology , heart failure , biochemistry , phosphorylation , chemistry , signal transduction
Cardiovascular disease is among the major causes for increased morbidity and mortality rates. High alcohol consumption may lead to cardiomyopathy, cardiac arrhythmias, and a suite of other disorders. Previous studies in our lab have linked the Akt/PI3K (serine/threonine kinase/ phosphoinositide 3‐kinase) and mitogen‐activated protein kinase (MAPK) pathway to cardiovascular disease. Creatine acting as an energy source in the cell can activate mTOR which is a mediator in the Akt/PI3K pathway which is involved in activating stress relief signals. In this study, since we know that alcohol is a stressor in the cardiovascular system, we hypothesize that high creatine can abrogate the stress of alcohol. We tested cardiac tissue perfused with low and high alcohol and two levels of creatine (30 µM and 100 µM). Our data shows a 133% decrease in ERK1/2 activity with high alcohol and 30 µM creatine. However, ERK1/2 activity shows a 74% increase with high alcohol and 100 µM creatine. P38 expression showed a general decrease with high alcohol and both levels of creatine (30 µM and 100 µM) as compared to low alcohol with 30 µM creatine. These results suggest that the high creatine levels (100 µM) may not be abrogating the MAPKs expression; however, it seems to have a small effect on the p38 stress signal.