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Satellite cell activation and populations on single muscle‐fiber cultures from adult zebrafish ( Danio rerio ) (539.8)
Author(s) -
Zhang Helia,
Anderson Judy
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.539.8
Subject(s) - danio , zebrafish , microbiology and biotechnology , biology , hepatocyte growth factor , chemistry , receptor , biochemistry , gene
Satellite cell activation and populations on single muscle‐fiber cultures from adult zebrafish (Danio rerio) Helia Zhang and Judy E Anderson, Department of Biological Sciences, University of Manitoba, Canada Satellite‐stem cells in adult mouse skeletal muscle are activated for growth or regeneration by signaling via nitric oxide, hepatocyte growth factor (HGF) and the c‐met receptor. Here, the regulation of satellite cell activation in adult zebrafish was assessed using the model system of isolated fibers cultured at 27°C and 21°C. Satellite cells were identified c‐met+ staining; activation was determined by uptake of bromodeoxyuridine. Satellite cells were maximally activated by the NO‐donor isosorbide dinitrate (ISDN, 1mM) or HGF (10 ng/mL); levels lower and higher respectively, than in mouse fibers. HGF‐induced activation was blocked by anti‐c‐met antibody, and reduced at 21°C. Cyclical stretch (3 hr at 4 cycles per min) increased activation; NOS inhibition and culture at 21°C reduced stretch activation. Stretching rapidly increased the number of c‐met+ cells/fiber (by 3 hr). Satellite cell activation signaling on zebrafish fibers, in particular temperature‐dependent responses to HGF and stretch, give new insights into the influences of ectothermy in regulating muscle growth and suggest the single‐fiber model system can be used to explore the basis of fiber hyperplasia in teleosts. Grant Funding Source : Supported by Natural Sciences and Engineering Research Council