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WNT1‐inducible protein 1 regulates intestinal mucosal wound resealing (488.9)
Author(s) -
Nishio Hikaru,
Neumann Philipp,
Leoni Giovanna,
Quiros Miguel,
Bernal Gabriela,
Feng Mingli,
Parkos Charles,
Nusrat Asma
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.488.9
Subject(s) - matricellular protein , wnt signaling pathway , microbiology and biotechnology , wound healing , epithelium , intestinal epithelium , inflammation , downregulation and upregulation , chemistry , caco 2 , cancer research , signal transduction , in vitro , biology , immunology , extracellular matrix , pathology , medicine , biochemistry , gene
The intestinal epithelium has a remarkable capacity to migrate and proliferate to reseal wounds and re‐establish the epithelial barrier under conditions of inflammation. We report that a matricellular protein transcriptionally regulated by WNT/β‐catenin pathway referred to as WNT1‐inducible signaling protein 1 (WISP1) is upregulated in resealing colonic epithelial wounds in vitro and in vivo. Similarly, we observed increased WISP1 protein and mRNA expression in the intestinal epithelium of patients with active inflammatory bowel disease. Exogenous administration of WISP 1 promoted wound closure of model intestinal epithelial cell lines in vitro through increased epithelial proliferation and cell migration. The beneficial effects of WISP1 on wound closure were mediated by activation of focal adhesion kinase and c‐Src‐mediated signaling. These findings highlight a mechanism by which a WNT/β‐catenin target protein released by injured epithelial cells promotes mucosal wound closure.