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Modularity in nuclear mechanotransduction and genome regulation (479.3)
Author(s) -
Shivashankar G.V.
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.479.3
Subject(s) - mechanotransduction , modularity (biology) , biology , microbiology and biotechnology , extracellular matrix , neuroscience , functional genomics , cell mechanics , computational biology , genomics , genome , cell , gene , evolutionary biology , genetics , cytoskeleton
Mechanical signals from the extracellular matrix impinge on cellular geometry resulting in altered functional nuclear landscape and gene expression programs. These alterations regulate diverse biological processes including stem‐cell differentiation, developmental genetic programs and cellular homeostasis. How such signals are integrated to the 3D spatio‐temporal organization of the cell nucleus to elicit differential gene expression patterns are poorly understood. To investigate the biophysical principles underlying these processes, we use a multi‐disciplinary approach, combining high resolution imaging of live‐cells cultured on micro‐patterned substrates, single‐cell mechanics and genomics. I will describe some of our ongoing work that is beginning to provide quantitative and modular links between cell geometry and nuclear mechanics in regulating 3D chromosome organization and genetic information.