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Organ engineering using decellularized liver scaffold recellularized with iPSC‐derived hepatocytes (398.10)
Author(s) -
Kanagavel Vidhya,
Ren Songyang,
Ignatius Irudayam Joseph,
Xiong Weidong,
Talavera Dodanim,
Klein Andrew,
French Samuel,
Arumugaswami Vaithi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.398.10
Subject(s) - decellularization , induced pluripotent stem cell , scaffold , liver transplantation , extracellular matrix , transplantation , tissue engineering , bioartificial liver device , progenitor cell , microbiology and biotechnology , stem cell , pathology , chemistry , embryonic stem cell , biology , biomedical engineering , medicine , hepatocyte , in vitro , biochemistry , gene
Liver transplantation is the option for end‐stage liver diseases. Shortage of donor liver organ is a major issue. To overcome this, alternative approaches including hepatic cell transplantation, bioartificial liver devise and whole liver organ engineering are being investigated. We focus on developing engineered liver using natural liver scaffold generated via decellularization process. The liver scaffold would maintain the authentic three‐ dimensional micro‐architecture and vasculature. The liver scaffold repopulated with patient‐specific hepatic cells differentiated from induced pluripotent stem cells (iPSC) is a promising option. For this proof‐of‐concept study, we decellularized rat liver by perfusing detergents SDS and Triton x‐100. Histological analysis of decellularized liver matrix (DLM) revealed removal of cellular component and preservation of extra‐cellular architecture. Presence of collagen type IV and laminin in the DLM was verified. Human endothelial cells seeded through DLM portal vein present in the vascular linings. We have also established an efficient protocol for deriving functional hepatic cells from iPSC. Perfusion of DLM with green fluorescent protein labeled iPSC‐hepatocytes resulted in recellularization of liver parenchymal space. Currently, we are assessing the in situ functions of repopulated iPSC‐hepatocytes. In conclusion, we have generated natural liver scaffold from rat that can be populated with human iPSC‐hepatocytes and endothelial cells.