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Human beta‐defensins‐1, ‐2, and ‐3 production by human amniotic epithelial cells in relation to human papillomavirus genotype (397.4)
Author(s) -
Szukiewicz Dariusz,
Alkhalayla Habib,
Stangret Aleksandra,
Szewczyk Grzegorz
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.397.4
Subject(s) - beta defensin , biology , genotype , immune system , virology , hpv infection , immunology , innate immune system , microbiology and biotechnology , gene , cervical cancer , cancer , genetics
HPV is common sexually transmitted infection. Based on the oncogenic potential, low‐risk and high‐risk HPV types were distinguished among approximately 40 HPV genotypes identified within the mucosa of the anogenital tract. Epithelial HBDs act as a potent protein adjuvants promoting cellular and humoral immune responses in viral infections. Unlike alpha‐defensins, HBDs are upregulated in HPV infections. We compared strenght of this response in respect to oncogenic (types 16 and 18) and non‐oncogenic (6 and 11) HPVs (groups I and II, respectively). After normal pregnancies (N = 96) HAEC were isolated from the amnions using trypsinization and divided into three groups (including negative controls; group III), according to the results of the vaginal smear real‐time PCR test for detection of HPV DNA. HAEC stimulated with lipopolysaccharide (1μg/ml) were cultured in vitro for 5 days. HBD‐1, HBD‐2, and HBD‐3 were quantified by ELISA in the culture media samples. Observed mean increases for HBDs were 5.7‐, 3.4‐, and 4.6‐fold, compared to the controls. The mean increases in HBDs concentrations were weaker in the oncogenic HPV infection, compared to the non‐oncogenic HPVs, and the differences between group I and II reached statistical significance (p < 0.05) for HBD‐1 and HBD‐3. Thus, HPV‐related HBD overproduction depends on the virus genotype. Subdued response to oncogenic HPVs may be included in their pathomechanism. Grant Funding Source : Supported by WUM grant: 2M2‐W2‐13.