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Granulocyte/monocyte phagocytosis is related to inflammation and disease risk factors in overweight/obese women (392.2)
Author(s) -
Nieman David,
Dréau Didier,
Henson Dru,
Luo Beibei,
Meaney Mary Pat,
Shanely R. Andrew,
Dew Dustin
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.392.2
Subject(s) - medicine , overweight , oxidative stress , insulin , immunology , endocrinology , systemic inflammation , respiratory burst , monocyte , inflammation , obesity
Correlates of granulocyte (GPHAG) and monocyte (MPHAG) phagocytosis and oxidative burst activity (GOBA, MOBA) were investigated during a 12‐week period in overweight/obese women (N=106, 18‐75 years, BMI 蠅 25 kg/m 2 ). Blood samples were collected pre‐ and post‐study, and analyzed for oxidative stress/capacity (oxidized LDL, RBC glutathione, 8 hydroxy‐2‐deoxyguanosine, FRAP, ORAC), inflammation (CRP, WBC differential), and metabolic factors (insulin, hemoglobin A1C, human growth hormone, epinephrine, cortisol, lipid/diagnostic chemistry panel). GPHAG, MPHAG, GOBA, and MOBA were measured using flow cytometric methods and Staphylococcus aureus as the target pathogen (bacteria‐to‐phagocyte ratio of 8:1). The Wisconsin Upper Respiratory Symptom Survey was used to assess acute respiratory illness (ARI). Pre‐ and post‐study blood sample outcomes were averaged and correlated with GPHA, MPHAG, GOBA, and MOBA. GPHAG and MPHAG (but not GOBA or MOBA) were significantly related with the neutrophil/lymphocyte ratio (NE/LY) (r=0.62, 0.61, respectively, P<0.001), BMI (r=0.50, 0.40, P<0.001), CRP (r=0.44, 0.30, P<0.01), insulin (r=0.30, 0.23, P<0.03), and A1C (r=0.28, 0.21, P<0.04), but not with ARI, oxidative stress/capacity, or other metabolic factors. In summary, GPHAG and MPHAG were related to NE/LY, BMI, and CRP in overweight/obese women and can be regarded as biomarkers of systemic inflammation.