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Gamma tocotrienol improves high fat diet‐induced obesity and insulin resistance by inhibiting adipose inflammation and macrophage recruitment (383.4)
Author(s) -
Zhao Lu,
Kang Inhae,
Okla Meshail,
Ha JungHeun,
Lee Meeae,
Marshall Maurice,
Chung Soonkyu
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.383.4
Subject(s) - adipose tissue , endocrinology , medicine , insulin resistance , adipocyte , inflammation , adipose tissue macrophages , proinflammatory cytokine , insulin , macrophage polarization , macrophage , biology , biochemistry , in vitro
Childhood obesity is associated with an abnormal increase of adipocyte hyperplasia. We have previously reported that gamma tocotrienol (γT3) potently inhibits adipocyte hyperplasia in human adipose‐derived stem cells. To investigate γT3 on childhood obesity, young C57BL/6J mice were fed a high fat (HF) diet containing 0.05% γT3 for 4 wks. γT3 accumulated in adipose tissue and reduced weight gain specifically in epididymal fat. γT3‐fed mice were associated with 1) decreased plasma levels of fasting glucose, insulin, and proinflammatory cytokines, 2) improved glucose tolerance, and 3) enhanced insulin signaling in adipose tissue. There was a substantial decrease of F4/80, a macrophage surface marker, and MCP1 expression suggesting that γT3 reduces recruitment of adipose tissue macrophage (ATM). Additionally, we hypothesized that γT3 sensitizes insulin by decreasing inflammation. γT3 treatment in adipocytes resulted in 1) a significant suppression of MAP kinase and NFκB activation, and 2) restoration of insulin‐stimulated glucose uptake against inflammation. In parallel, γT3 reduced LPS‐mediated M1 macrophage polarization in bone marrow stem cells. Taken together, our results demonstrated that γT3 ameliorates HF diet‐mediated visceral obesity and insulin resistance by inhibiting systemic and adipose inflammation, and ATM recruitment. Grant Funding Source : Supported by American Heart Association