Premium
The role of the probiotic Bifidobacterium animalis subsp. lactis on systemic immune function in humans (382.7)
Author(s) -
Meng Huicui,
Roberts Robert,
KrisEtherton Penny,
Lee Yujin,
Ba Zhaoyong,
Fleming Jennifer,
Furumoto Emily,
Rogers Connie
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.382.7
Subject(s) - bifidobacterium animalis , immune system , probiotic , peripheral blood mononuclear cell , bifidobacterium , immunity , immunology , biology , innate immune system , acquired immune system , microbiology and biotechnology , t cell , bacteria , lactobacillus , in vitro , biochemistry , genetics
Evidence suggests that probiotic bacteria modulate both innate and adaptive immunity; however the immune response varies by type of organism. Probiotics in the genera Bifidobacterium may stimulate T cell and cytokine responses that augment cell mediated immunity. The goal of the current study was to evaluate the effect of one species, Bifidobacterium animalis subsp. lactis BB‐12 at a dose of log 10 ± 0.5 CFUs/day on immune responses in a controlled, 4‐period crossover, free‐living study. Furthermore, the matrix used to deliver probiotic bacteria may influence the performance and efficacy of probiotic interventions in vivo . Therefore, a sub‐aim of this project will determine if the immune response to BB‐12 differs depending on the delivery matrix of the probiotic bacteria. Healthy adults (n=27) aged 18‐40 years old with BMI 20‐35 kg/m 2 were recruited, and received 4 treatments in a random order: 1) yogurt smoothie alone; smoothie with organism added 2) before or 3) after fermentation, or 4) organism given in tablet form. At baseline and after each treatment, peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood, and functional and phenotypic marker expression was assessed. The distribution of immune cell subsets (e.g. T and B lymphocytes, nature killer cells, dendritic cells, macrophages and granulocytes) in peripheral blood was not altered by any treatment However, we observed a treatment effect on TNF‐α and IL‐6 secretion from BB‐12 (p=0.027) and LPS stimulated PBMCs and on T cell proliferation (p=0.036). This interim analysis suggests that probiotic treatment may be altering the immune response in humans, and the delivery matrix of BB‐12 also influences its performance in vivo . Grant Funding Source : Supported by Dairy Research Instituite