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EPA and DHA increase the frequency of murine B cell subsets and restore antibody production in obesity upon stimulation with a T‐independent antigen (382.5)
Author(s) -
Teague Heather,
Harris Mitchel,
Shaikh Saame
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.382.5
Subject(s) - fish oil , docosahexaenoic acid , eicosapentaenoic acid , stimulation , b cell , t cell , lipopolysaccharide , in vivo , endocrinology , immune system , medicine , chemistry , biology , antibody , fatty acid , immunology , biochemistry , polyunsaturated fatty acid , fish <actinopterygii> , microbiology and biotechnology , fishery
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the bioactive components of fish oil, exert immunomodulatory properties. The impact of EPA and DHA supplementation on murine B cell function is poorly studied. We first determined the effects of fish oil on B cell phenotypes and antibody production in obese mice upon stimulation with a T‐independent antigen. Fish oil intervention increased the frequency of transitional 1 and marginal zone B cells in obese mice. Fish oil restored the decrement in circulating IgM in obesity and increased antibody production in lean mice. We then measured the effects of EPA and DHA ethyl esters on B cell activation upon supplementation to an obesogenic diet. Ex vivo stimulation of B cells with lipopolysaccharide increased B cell activation as measured by TNF‐α and IL‐6 secretion. DHA, but not EPA, increased B cell IL‐10 production. In vivo, EPA and DHA differentially increased the frequency of transitional 1, transitional 2 and pre‐marginal zone cells. Taken together, the data establish that fish oil can boost B‐cell mediated immunity in obesity and that EPA and DHA ethyl esters exert differential effects on B cells.