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Ganoderma lucidum ReishiMax restored aging‐related changes in expressions of multiple gene pathways in normal aging mice (373.6)
Author(s) -
Tan Ningzhi,
Gao Ling,
Xu Chi,
Yang Jieying,
Zhang Yan,
Zhao Chunsheng,
Dong Yunzi,
Lu Jihong,
Han Jacky,
Zhu JiaShi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.373.6
Subject(s) - gene , microarray , gene expression , immune system , spleen , pi3k/akt/mtor pathway , signal transduction , biology , cancer research , microbiology and biotechnology , chemistry , immunology , biochemistry
Ganoderma lucidum (Reishi) is traditionally used as a medicinal herb in China for immune enhancement, cancer prevention and adjuvant therapy. We reported that ReishiMax (RM) with enrichment of Reishi triterpenes and polysaccharides extends the lifespan of mice (FASEB J 2012, 26:373.2). We further studied molecular mechanisms of RM’s anti‐aging effect in aged mice. Aging‐induced changes in gene pathway expressions (GE) were tested via microarray on the liver, spleen, muscle and neocortex from young (5 mo), old (25 mo) and old RM treated (0.175 g/kg) mice (n=16 each). With a setting of FDR q<0.01, aging related changes in gene sets were noted as differentially expressed GOs (DEG). RM reversed those DEG, 842 of them up‐regulated and 229 down‐regulated. DEG with aging and after RM were correlated negatively: remarkably in muscle PCC= ‐0.82, or in spleen ‐0.93, but moderately in liver ‐0.53 or in neocortex ‐0.55. Cancer and metabolism pathways were highlighted. GESA analysis showed that RM down‐regulated TGF‐beta signaling pathway via altered expressions of 2 sets of transcriptional factors. In conclusion, our study shows that RM reverses multiple aging‐related changes in GE as the molecular mechanisms of the lifespan extending effects of RM. RM down‐regulates the TGF‐beta signaling pathway, which may play an important role in anti‐aging.