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Comparison of anti‐inflammatory mechanisms of mango ( Mangifera indica L.) and pomegranate ( Punica granatum L.) in DSS‐induced colitis in rats (372.8)
Author(s) -
Kim Hyemee,
Banerjee Nivedita,
Ivanov Ivan,
Talcott Stephen,
MertensTalcott Susanne
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.372.8
Subject(s) - mangifera , punica , pi3k/akt/mtor pathway , colitis , polyphenol , inflammation , ulcerative colitis , chemistry , pharmacology , traditional medicine , signal transduction , medicine , biochemistry , biology , immunology , botany , antioxidant , disease
Ulcerative colitis, a chronic inflammation of the large intestine, may increase risk of human colorectal cancer. Several studies have focused on developing natural anti‐inflammatory products. Polyphenolics from mango (mainly gallotannins) and pomegranate (mainly ellagitannins) have been shown to have potent anti‐inflammatory properties. To determine the anti‐inflammatory effects and possible mechanisms of mango and pomegranate juice in DSS‐induced colitis, SD rats were administered control, mango, or pomegranate juice, and were exposed to three cycles of 3% DSS followed by 2‐week recovery period. The levels of protein involved in the mTOR pathway were analyzed by multiplex bead assay, while the transcriptional variation with the mTOR pathway were analyzed by low density PCR arrays. In addition, the levels of several miRNAs involved in the mTOR pathway were measured. Chronic colitis was caused by DSS in rats, and mango and pomegranate juice reduced colon inflammation compared to control juice. Mango juice suppressed the IGF‐1R‐AKT/mTOR signaling axis via up‐regulation of miR‐126 and down‐regulation of IR, while pomegranate decreased p70S6K by up‐regulating miR‐145 and down‐regulation of the MEK‐ERK1/2 pathway. These results suggest that polyphenolics of different predominant structure may differentially regulate inflammation‐involved pathways while attenuating DSS‐induced colitis.