Premium
Genistein promotes a gene expression profile characteristic of brown rather than white adipocytes and increases Sirt1 expression in mouse NIH3T3‐L1 cells (372.7)
Author(s) -
Aziz Sadat,
Wakeling Luisa,
Hesketh John,
Ford Dianne
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.372.7
Subject(s) - genistein , adipogenesis , white adipose tissue , endocrinology , medicine , prdm16 , resistin , resveratrol , adipocyte , adipose tissue , biology , fatty acid synthase , gene expression , thermogenin , sirtuin , gene , adipokine , lipid metabolism , biochemistry , obesity , leptin , acetylation
Various possible benefits have been attributed to the dietary isoflavone genistein. In common with resveratrol, which may act via sirtuin 1 (Sirt1), these benefits include protection from diet‐induced obesity. We thus aimed to determine if genistein affected adipogenesis and/or Sirt1 expression using a cell culture model. NIH3T3‐L1 cells were differentiated into adipocytes (from 48 h post‐confluence, taken as day 0) in the presence or absence of genistein. Gene expression was measured by RT‐qPCR. A low concentration of genistein (10 µM) and/or shorter exposure (days 0‐12) promoted differentiation to white adipocytes, indicated by large fat droplets and increased expression of adipocyte marker genes ( Acaca (acetyl Co‐A carboxylase‐α) , Fasn (fatty acid synthase) , Fabp4 (fatty acid binding protein 4) , Lipe (hormone sensitive lipase), Retn (resistin), Rarres2 (chemerin)). However, at higher concentrations of genistein (50‐100 µM) and/or after longer exposure (days 0‐12) cells had smaller fat droplets and lower expression of these genes, coupled with increased expression of Sirt1 and of genes characteristic of brown adipocytes ( Ucp1 (uncoupling protein 1) and Cebpb (CCAAT/enhancer binding protein‐β)). Dietary genistein may thus protect against obesity by encouraging the development of brown, rather than white, adipose tissue, possibly through a mechanism involving Sirt1. Grant Funding Source : Kurdistan Regional Government