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The effect of thermogenic and appetite suppressing capsules on mice fed a high fat diet (371.2)
Author(s) -
DiSilvestro David,
Lee L James,
Ziouzenkova Ouliana
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.371.2
Subject(s) - medicine , endocrinology , appetite , basal metabolic rate , weight loss , leptin , capsule , thermogenesis , fat pad , adipose tissue , chemistry , obesity , biology , botany
Obesity affects approximately 30% of Americans. Novel treatment methods are needed when traditional methods, like diet and exercise, fail. Increasing the basal metabolic rate through increased thermogenesis should result in weight loss through increased heat production. However, the treated subject may compensate for the weight loss with increased food consumption. Furthermore, therapies that implant unprotected thermogenic cells into fat may induce the host’s immune response. We proposed a solution to these problems by encapsulating thermogenic and potentially appetite suppressing cells in a protective porous alginate‐poly‐l‐lysine membrane. We generated a thermogenic cell line by deleting the regulatory associated protein of mTOR, in 3T3‐L1 pre‐adipocytes (RKO). We stably transduced these cells with the genes for the appetite suppressing hormones, leptin and amylin, to generate the LAR cell line. We fed 12 week old wild‐type female mice a high‐fat diet (45% kcal from fat) for 132 days and treated them with cellular or acellular/empty (E) capsules. The control group (EV‐ES) received acellular capsule injections into both subcutaneous (S) and visceral (V) fat pads. Another group (RV‐ES) received RKO capsule injections into the V fat pads and acellular capsule injections into the S fat pads. The final group (RV‐LAR_S) received RKO capsules into the V fat pads and LAR capsules into the S fat pads. At 45 days post injection, the normalized weight was less in RV‐LAR_S than in control EV‐ES mice. RV‐LAR_S also exhibited a higher resting metabolic rate as measured by heat production per kg when compared to the other groups, but glucose tolerance tests yielded similar results for all groups. The food intakes for all groups were the same. The limited capsule efficacy may be due to a limited number of encapsulated cells surviving months after injection. Our results show the efficacy of encapsulated thermocytes in increasing thermogenesis and attenuating weight gain induced by a HF diet.