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Mechanistic insights on the preprotein translocase: a single molecule study (362.4)
Author(s) -
Driessen Arnold
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.362.4
Subject(s) - translocase , heterotrimeric g protein , membrane transport protein , translocon , microbiology and biotechnology , transport protein , ribosome , motor protein , biophysics , cytoplasm , chemistry , membrane protein , biology , chromosomal translocation , biochemistry , membrane , g protein , rna , gene , signal transduction , microtubule
The heterotrimeric SecYEG complex comprises a protein‐conducting channel in the bacterial cytoplasmic membrane. SecYEG functions together with the motor protein SecA in preprotein translocation. SecYEG can also associate with a translating ribosome to mediate membrane protein insertion. We have used single molecule techniques, fluorescence spectroscopy and advanced membrane protein reconstitution methods to address mechanistic and structural questions on the protein translocase with the challenge to visualize single protein translocation events.