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Synthetically rerouting phagocytosis by rapidly turning inert cells into “eat you” mode (345.1)
Author(s) -
Inoue Takanari
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.345.1
Subject(s) - phagocytosis , jurkat cells , microbiology and biotechnology , hela , cell , effector , biology , chemistry , biophysics , immune system , t cell , immunology , biochemistry
Through ceaseless communications with the environment, cellular surface represents an origin of information flow that determines diverse cellular processes. Techniques to rapidly engineer the surface property of living cells should thus offer a power to harness the cellular functions. In this study, we developed and implemented a technique to induce presentation of a molecule of interest at the cell surface on a timescale of minutes. While the molecular mechanism of phagocytosis is well characterized, minimal molecular events that are sufficient to trigger this elaborate process remain elusive. With an induced surface display of a C2 domain of MFG‐E8 protein as well as Rac small GTPase activation inside cells, we rapidly turned inert human HeLa cells into “eat you” mode and made them bind and engulf apoptotic human Jurkat T cells. Actuation of neither signal was sufficient on its own to trigger phagocytosis, indicating target cell attachment and actin reorganization constitute minimal molecular events for fulfillment of phagocytosis. The present surface display technique may be used as a targeted cell‐based therapy where unwanted cells with characteristic surface molecules will be rapidly eliminated by engineered cells.