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Site‐and promoter‐specific epigenetic alterations by a common dietary compound (271.5)
Author(s) -
Dey Moul,
Liu Yi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.271.5
Subject(s) - epigenetics , acetylation , histone , cancer research , cancer , cruciferous vegetables , biology , chemistry , medicine , biochemistry , gene , genetics
Effects of dietary compounds on site‐specific histone modifications, which are key epigenetic regulators of gene expression impacting cancer etiology, remain relatively unexplored. Phenethylisothiocyanate (PEITC) is a common dietary compound derived from cruciferous vegetables. Our previous research have shown promising anti‐inflammatory and chemo preventive properties of PEITC. The present study reports novel, dynamic, site‐specific chemical changes to histone H3 in a gene‐promoter‐specific manner, associated with PEITC exposure in human colon tumor‐derived epithelial cells. The time‐and concentration‐dependent effects of PEITC on histone modifications (di‐and tri‐methylations of lysine‐9 and lysine‐27 and acetylation) and gene expression changes were achieved at low, non‐cytotoxic concentrations, in contrast to the higher concentrations necessary to halt cancer cell proliferation likely mediated by caspase‐dependent apoptotic signaling. Increased understanding of specific epigenetic alterations by dietary compounds may provide molecular predictors for improved preventive strategies for reducing the healthcare burden of cancer and other human diseases. Grant Funding Source : The work is supported by the National Institutes of Health grant R00AT4245 and SDAES grant 328100/31