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Carbohydrate feeding and impact on global metabolomics in relation to insulin sensitivity in men with metabolic syndrome (248.8)
Author(s) -
Watkins Bruce,
Pappan Kirk,
Kim Jeffrey,
Freidenreich Daniel,
Kunces Laura,
Volk Brittanie,
Saenz Catherine,
Volek Jeff
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.248.8
Subject(s) - medicine , endocrinology , insulin resistance , insulin , carbohydrate , metabolomics , metabolic syndrome , chemistry , carbohydrate metabolism , biology , diabetes mellitus , chromatography
Restriction in dietary carbohydrate (CHO) for several weeks results in a metabolic state where fatty acids and ketones become primary fuels, which correlates with improved insulin sensitivity and management of type 2 diabetes. To gain a broader understanding of the metabolic correlates to dietary CHO manipulation in relation to insulin sensitivity we performed serum global metabolomic analysis in men with metabolic syndrome who were fed calorically‐controlled diets for 21 d that incrementally increased CHO and decreased fat. The diets (isocaloric and isonitrogenous) consisted of low (LC), moderate (MC) and high (HC) CHO levels corresponding to 47, 131, and 345 g CHO/d. HOMA insulin resistance decreased by 50% from baseline (BL) to LC (3.0 to 1.5) and increased after MC (1.7) and HC (2.0). Change in HOMA from BL to LC was positively correlated to changes in butyrylcarnitine, gamma‐glutamyltyrosine, threobromine, and tyrosine, and inversely with α‐ketobutyrate and andro steroid monosulfate 2. The change in HOMA from LC to HC was positively correlated to 4‐hydroxyphenylpyruvate, alanine, arabinose, aspartate, gamma‐glutamyltyrosine, N6‐acetyllysine, and palmitoyl sphingomyelin, and inversely correlated with methyl‐β‐glucopyranoside. In summary, several novel metabolic markers were identified that may help explain the variable response in insulin sensitivity to dietary CHO restriction and refeeding.