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Dietary DHA supports glucose use by muscle in association with improved endocannabinoid system gene expression in C57/blk6 mice (248.7)
Author(s) -
Kim Jeffrey,
Pedersen Theresa,
Newman John,
Watkins Bruce
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.248.7
Subject(s) - endocannabinoid system , medicine , endocrinology , docosahexaenoic acid , glut4 , receptor , arachidonic acid , type 2 diabetes , chemistry , insulin , biology , glucose transporter , diabetes mellitus , fatty acid , enzyme , biochemistry , polyunsaturated fatty acid
Elevated levels of endocannabinoid system (ECS) ligands have been associated with increased adiposity and obesity, and are likely modulators of type II diabetes pathogenesis. High CB1 receptor expression and circulating arachidonic acid (AA)‐derived endocannabinoids (EC) have been found in both obese and diabetic patients. We wanted to determine whether dietary docosahexaenoic acid (DHA) could influence endocannabinoid tone, which includes the action of ligands, receptors, and EC synthesis and degradation enzymes, to prevent an overactive ECS whilst improving aspects of euglycemia. To assess this, male C57bl/6 mice were fed a semi‐purified diet containing DHA or control for 118 d. Serum, gastrocnemius, epididymal fat pads, and liver were collected after 62 and 118 d of feeding. DHA diet fed mice had lower epididymal fat pad masses compared to the control diet group. A compensatory response resulted in DHA fed mice with higher EC receptors and synthesis/degradation enzyme mRNA expression. However, AA‐derived EC (2‐AG and AEA), and downstream ECS activation markers were reduced in DHA fed mice at both 62 and 118 d, despite time dependent increases in these EC. Mice fed the DHA diet had higher glucose‐related ( GLUT4 & Insulin‐R ) mRNA and proteins. Collectively our findings demonstrate that a DHA diet fed to mice resulted in a reduction in ECS activation leading to an increase in glucose uptake markers and lipolysis.