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Plasma metabolomic profiles associated with hospital mortality in surgical critical illness (248.2)
Author(s) -
Frediani Jennifer,
Park Youngja,
Hebbar Gautam,
May Addison,
Wischmeyer Paul,
Sax Harry,
Kudsk Kenneth,
Uppal Karan,
Jones Dean,
Ziegler Thomas
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.248.2
Subject(s) - metabolite , metabolomics , medicine , metabolite profiling , glutamine , demographics , intensive care unit , metabolome , critical illness , pharmacology , bioinformatics , biology , demography , critically ill , biochemistry , amino acid , sociology
Background: Plasma metabolomic profiling provides an opportunity for discovery‐based studies on human metabolism during illness. We sought to define metabolites that differ between intensive care unit (ICU) patients that did or did not survive hospitalization. Methods: Subjects matched on demographics and nutrient administration in a RCT on glutamine supplementation in surgical ICU patients were selected based on hospital mortality. Serial plasma was analyzed using high‐resolution LC‐MS methods. False discovery rate (FDR; q=0.05) and hierarchal cluster analysis was used to distinguish accurate mass ions (metabolites) significantly different between groups. The METLIN Metabolite Database was used to match ions to known metabolites. Results: A total of 22,305 metabolites were detected (54, 5, 33, and 113 were significantly different between the two groups at days 0, 3, 7, and 14, respectively). Survivors and non‐survivors were differentiated at both day 0 and day 14 by 3 metabolite matches (2 unknowns and stearic acid), and by several others at days 7 and 14 (2 unknowns, terpenoids, drug metabolites). Several drug metabolite matches were higher and several endogenous metabolites were lower in non‐survivors, respectively. Conclusion: High‐resolution LC‐MS can identify plasma metabolomic profiles associated with hospital mortality in adults with surgical critical illness. Grant Funding Source : Supported by grants from the National Institutes of Health Fogarty grants D43 TW007124