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Nuclear transport and gene expression (238.3)
Author(s) -
Weis Karsten
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.238.3
Subject(s) - nuclear pore , messenger rna , microbiology and biotechnology , nuclear export signal , p bodies , gene expression , chemistry , biology , translation (biology) , cytoplasm , gene , cell nucleus , biochemistry
Nuclear export is an essential step in the expression of every eukaryotic mRNA. However, it remains poorly understood how mRNAs are unidirectionally transported across the nuclear envelope and how this process is regulated. The DEAD‐box ATPase Dbp5/DDX19 functions in mRNA export and is thought to remove mRNA export factors from the message to terminate the translocation through the nuclear pore complex (NPC). Dbp5 is localized to the NPC via an interaction with Nup159/Nup214 and is locally activated there by Gle1 together with the small‐molecule inositol hexakisphosphate (IP 6 ). Local activation of Dbp5 at the NPC by Gle1 is essential for mRNA export in vivo but the mechanistic role of Dbp5 in mRNP export remains unlcear. We have addressed the question of how Dbp5 contributes to directional mRNA export and have analysed the function of Dbp5 biochemically, structurally and by single molecule experiments. Once mRNAs reach the cytoplasm, they can be either translated, stored or decayed. An important regulator of the transition between mRNA translation and decay is the DEAD‐box ATPase Dhh1/DDX6. We have shown that recruitment of Dhh1 to an mRNA is sufficient to move this mRNA from an active state to translational repression and to localize it into processing bodies (P bodies). Our research has focused on the question of how the ATPase function of Dhh1 is used to control whether an mRNA is translated, stored or decayed.