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Deep methylomes reveal diverse roles of DNA methylation in human tissues (235.1)
Author(s) -
Ecker Joseph,
Schultz Matthew,
He Yupeng,
Hariharan Manoj,
Whitaker John,
Nery Joseph,
Urich Mark,
Chen Huaming,
Leung Danny,
Rajagopal Nisha,
Lin Shin,
Lin Yiing,
Snyder Michael,
Wang Wei,
Ren Bing
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.235.1
Subject(s) - dna methylation , epigenome , biology , methylation , genome , differentially methylated regions , genetics , epigenomics , computational biology , dna , gene , gene expression
In mammals, DNA methylation is a well‐known contributor to cellular identity and is required for normal development. A thorough mapping of the diversity of methylation patterns is a key step in understanding their role in establishing the identity of different tissues. We have produced high coverage methylomes, transcriptomes, and genomes of 18 different tissue types from 4 human donors. These data revealed a large set of differentially methylated regions, many of which are tissue‐specific and identify regions important for tissue function. In addition to locus specific changes, these methylomes revealed partially methylated domains in pancreas, which covered a significant fraction of its genome and are transcriptionally repressed. We found genome‐wide evidence for nonCG methylation in a subset of these tissues, a finding that had previously been restricted to embryonic stem cells and brain tissue. These results extend our knowledge of the unique role of DNA methylation in organ development and function, and offer a new framework for testing the role of the epigenome in healthy function and in pathological states in each of the major organ systems.