Premium
Mixed proteome analysis for clarification of the mechanism of infectious candidiasis (152.6)
Author(s) -
Kitahara Nao,
Morisaka Hironobu,
Aoki Wataru,
Kuroda Kouichi,
Ueda Mitsuyoshi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.152.6
Subject(s) - candida albicans , corpus albicans , macrophage , proteome , microbiology and biotechnology , phagocytosis , pathogen , biology , chemistry , fungus , systemic candidiasis , virulence , biochemistry , in vitro , botany , gene
Candida albicans is an opportunistic pathogen that causes fatal disease in immunocompromised hosts. Host resistance against pathogenic fungus such as C. albicans relies on the ingestion and elimination by macrophage. However, once inside the macrophage, C. albicans can break through it and spread around the body. In this study, using mixed proteome analysis, we have investigated the response of C. albicans to phagocytosis by macrophage. Unlike previous works, mixed proteome analysis does not require any additional prefractionation of two organisms prior to the separation and detection by nanoLC‐MS/MS system. This system is equipped with a monolithic silica capillary column (500 cm long), which offers high separation efficiency to the tryptic peptides in highly complex samples [1,2]. We cultivated C. albicans with macrophages for 0, 3, and 5 h, and determined 499 and 1273 proteins for C. albicans and macrophage, respectively. Pathway analyses of C. albicans proteins and macrophage proteins were carried out. From these results, a model of the interaction of C. albicans with macrophage was constructed [3].