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Leukocyte specific protein‐1: a novel regulator of hepatoma proliferation and migration (144.10)
Author(s) -
Koral Kelly,
Paranjpe Shirish,
Mars Wendy,
Michalopoulos George
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.144.10
Subject(s) - small hairpin rna , cell growth , cyclin d1 , hepatocellular carcinoma , proliferating cell nuclear antigen , cancer research , gene knockdown , cell culture , regulator , biology , cancer , cell cycle , gene , genetics
Copy number variation analysis of human hepatocellular carcinoma samples revealed leukocyte specific protein‐1 (LSP1) gene had the greatest number of deletions. The role of LSP1 in the development or progression of hepatocellular carcinoma remains unknown therefore our objective was to determine the functional significance of LSP1 in liver cancer. Using shRNA against LSP1, we were able to create a stable cell line using the LSP1 expressing JM1 rat hepatoma cells. Analysis of proliferation and migration utilizing the MTT and scratch assay, respectively revealed that loss of LSP1 expression leads to increased proliferation and migration in the JM1 stable cell line as compared to scrambled shRNA control. The stably expressing LSP1 shRNA JM1 cells expressed higher levels of the proliferation markers, cyclin D1 and PCNA providing further evidence of a role for LSP1 in regulating the proliferation of hepatoma cells. Future studies will aim to examine if expression of LSP1 in a LSP1 deficient cell line will result in decreased proliferation and migration. Understanding the function of LSP1 in liver cancer may lead to novel therapeutic targets to combat this deadly disease. Grant Funding Source : Supported by NIH