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A polyherbal dietary intervention preserves functional islet β‐cell mass in non‐obese diabetic mice (134.8)
Author(s) -
Burke Susan,
Karlstad Michael,
Reel Danielle,
McEntee Michael,
Whelan Jay,
Collier James
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.134.8
Subject(s) - medicine , diabetes mellitus , nod mice , endocrinology , islet , chemokine , type 1 diabetes , nod , autoimmune disease , insulin , immunology , inflammation , disease
Type 1 diabetes mellitus results from autoimmune‐mediated destruction of pancreatic islet β‐cells. Dietary supplements that decrease blood glucose levels or prevent losses in functional β‐cell mass are currently sought for treatment of autoimmune‐mediated diabetes. We fed female non‐obese diabetic (NOD) mice, a model of spontaneous autoimmune diabetes, a diet supplemented with herbal extracts (32g total herbal extracts in 12kg of diet) over a 12 week period. The mice consumed isocaloric diets without (controls) and with polyherbal supplementation (PHS) ad libitum starting at a prediabetic stage (age 6 weeks) for 12 weeks. Control mice developed hyperglycemia (>180mg/dL) within 16 weeks (n = 8). By contrast, mice receiving the PHS diet did not develop hyperglycemia by 18 weeks (n = 8). After 18 weeks, there was a 31.9% increase in insulin‐positive cell mass in PHS mice relative to controls (n = 8). We also detected a 26% decrease in CD3 + lymphocytic infiltration in PHS mice relative to controls (n = 8). In vitro assays confirmed a 25% decrease in β‐cell expression of the chemokines CCL2 and CXCL10 with PHS media relative to controls. We conclude that daily polyherbal supplementation may be beneficial towards reducing severity or delaying time of onset of autoimmune‐mediated diabetes.