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Supplementation of polyphenol‐rich blackcurrant extract exerted hypolipidemic and anti‐inflammatory effects in diet‐induced obese mice (121.7)
Author(s) -
Benn Tyler,
Kim Bohkyung,
Park YoungKi,
Ku Chai Siah,
Yang Yue,
Pham Tho,
Wegner Casey,
Faruggia Callie,
Harness Ellen,
Lee JiYoung
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.121.7
Subject(s) - endocrinology , medicine , steatosis , inflammation , hyperlipidemia , adipose tissue , cholesterol , insulin resistance , lipopolysaccharide , adipocyte , cd68 , tumor necrosis factor alpha , obesity , chemistry , biology , immunohistochemistry , diabetes mellitus
Obesity is closely tied to hyperlipidemia, chronic inflammation, and insulin resistance. To gain insight into the effect of polyphenol‐rich blackcurrant extract (BCE) on obesity‐associated abnormalities, we fed 24 male C57BL/6J mice a high fat/high cholesterol diet (15% fat, 0.2% cholesterol by wt) supplemented with 0.1% BCE for 12 wk. Plasma total cholesterol and glucose were significantly reduced with marked increases in hepatic expression of sterol regulatory element binding protein 2 and low‐density lipoprotein receptor in BCE‐fed mice. Liver histology demonstrated less visible steatosis in BCE group. The body weight % of epididymal fat pads was significantly less in BCE‐fed mice compared to control. Furthermore, adipocyte size was smaller and the number of crown‐like structures, a marker of macrophage infiltration, was fewer in conjunction with decreased mRNA of the macrophage markers F4/80 and CD68 in epididymal adipose of BCE‐fed mice. Ex vivo analysis of splenocytes isolated from control and BCE‐fed mice showed that upon lipopolysaccharide stimulation, the expression of tumor necrosis factor α and interleukin 1 β were significantly reduced in the BCE group. In conclusion, BCE supplementation may be beneficial to prevent metabolic disturbance and chronic inflammation in obesity.