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Purinergic receptor stimulation induces Ca 2+ oscillations and smooth muscle contraction in small pulmonary veins (1175.6)
Author(s) -
Henriquez Mauricio,
PerezZoghbi Jose
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1175.6
Subject(s) - purinergic receptor , contraction (grammar) , suramin , extracellular , ryanodine receptor , phospholipase c , medicine , p2y receptor , intracellular , endocrinology , receptor , muscle contraction , stimulation , chemistry , biology , biophysics , microbiology and biotechnology
The small intrapulmonary veins (SPV) are thought to participate in the control of pulmonary blood flow and its excessive contraction has been associated with the development of pulmonary edema. However, the regulation of smooth muscle contraction by intracellular signaling in SPV is poorly understood. We used rat lung slices with phase‐contrast and confocal microscopy to investigate Ca 2+ signaling and contraction during purinergic receptor activation in small (100‐250 µm) pulmonary veins. We found that ATP induced strong, concentration‐dependent, and reversible vein contraction. Vein contraction was accompanied by intracellular Ca 2+ oscillations that propagated along the vein smooth muscle cell as Ca 2+ waves. In the absence of extracellular Ca 2+ , ATP induced Ca 2+ oscillations that were attenuated in time and transient vein contraction. The InsP 3 receptor inhibitor 2‐APB inhibited the Ca 2+ oscillations and contraction induced by ATP in absence of extracellular Ca 2+ . In contrast, Ca 2+ oscillations and contraction were resistant to ryanodine. Vein contraction was inhibited by phospholipase Cβ inhibitor U73122 and by purinergic receptor antagonist suramin. These results suggest that ATP is a strong vasoconstrictor of SPV and that ATP activate purinergic P2Y receptors in vein smooth muscle to induce InsP 3 receptor‐mediated Ca 2+ oscillations and vein contraction. Grant Funding Source : American Heart Association