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Solubleform of (pro)renin receptor is excreted from mesothelial cells to peritoneal dialysis effluent of peritoneal dialysis patients (1173.5)
Author(s) -
Oba Ikuko,
Mori Takefumi,
Takahashi Chika,
Ohsaki Yusuke,
Sato Emiko,
Koizumi Kenji,
Furusho Masahide,
Chida Makiko,
Naganuma Eri,
Ito Sadayoshi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1173.5
Subject(s) - peritoneal dialysis , mesothelial cell , flow cytometry , endocrinology , peritoneal equilibration test , in vitro , receptor , peritoneum , medicine , andrology , pharmacology , chemistry , biology , immunology , continuous ambulatory peritoneal dialysis , pathology , biochemistry
Renin angiotensin system (RAS) is associated with peritoneal injury in peritoneal dialysis (PD). Angiotensin II dependent and independent role of (pro) renin receptor ((P)RR) to the cellular injury has been demonstrated. Thus, the present study determined the expression of (P)RR in drained PD effluent (PDE) and human peritoneal mesothelial cells (HPMCs) of the PD patients. PDE drained after 2.5% glucose PD solution during peritoneal equivalent test (0hours, 2hours, 4hours) was collected from PD patients. Human soluble from of (P)RR level was measured by ELISA. The expression of (P)RR on cultured HPMCs and media isolated from PDE were investigated by Western blot analysis and/or flow cytometry. HPMCs was specified by cytokeratin positive cells in flow cytometry. Full length or soluble (P)RR was identified by the molecular weight compared to the full length and soluble (P)RR synthesized in vitro from the expression vectors. Soluble (P)RR was detected in both plasma and PDE of PD patients. Time dependent increase in soluble (P)RR was observed in PDE. Expression of (P)RR was observed in cytokeratin positive mesothelial cells. Full length (P)RR was detected in HPMCs while soluble from of (P)RR was detected in concentrated cultured medium. These results indicate that full length (P)RR is expressed in HPMCs and excrete soluble (P)RR in drained PDE. The role of full length (P)RR in HPMCs and soluble (P)RR in plasma and PDE of PD patients requires further investigation.