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Differential response of microRNA to contraction in aged and obese mice (1168.4)
Author(s) -
Rice Nicholas,
Haran Prashanth,
Fielding Roger,
Rivas Donato
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1168.4
Subject(s) - anabolism , skeletal muscle , microrna , endocrinology , medicine , contraction (grammar) , stimulation , insulin resistance , muscle contraction , downregulation and upregulation , biology , obesity , gene , genetics
Resistance to anabolic stimuli in skeletal muscle occurs within both aging and obese populations. However, the mechanisms responsible for this ‘anabolic resistance’ are unknown. MicroRNA (miRNA), small (20‐24 nucleotides) post‐transcriptional regulators, have recently been shown to play a role in the response of skeletal muscle to anabolic stimulation. We aimed to compare the response of miRNA to an acute bout of contraction via sciatic nerve electrical stimulation (ES) in skeletal muscle within three groups of C57BL/6 mice aged, 24 wks and fed a control (CON, 10% fat/kcal) or high‐fat (FAT, 60% fat/kcal) diet or aged 124 wks (OLD, 10% fat/kcal). The expressions of miRNA that are known (miR‐378, miR‐27a) and putative (miR‐126, miR‐324) regulators of skeletal muscle development were measured by qPCR at baseline (BL) and after ES. Obesity increased the BL expression of miR‐27a and miR‐324, while miR‐27a and mir‐378 expression was increased with age, compared to CON. After contraction, miR‐126 and miR‐378 were decreased in CON, while miR‐27a and miR‐324 were decreased in FAT. However, the only contraction‐induced change in OLD was a decrease of miR‐378 expression back to control levels. These results illustrate the differential responses of miRNA in skeletal muscle as a result of ES within these distinct groups. Grant Funding Source : USDA and NIH/NIA