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Skeletal muscle mitochondrial function in severely burned children improves following rehabilitative exercise (1159.13)
Author(s) -
Chao Tony,
Suman Oscar,
Herndon David,
Sidossis Labros,
Porter Craig
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1159.13
Subject(s) - skeletal muscle , oligomycin , respiration , mitochondrion , medicine , chemistry , endocrinology , biochemistry , anatomy , enzyme , atpase
Severe burn injury results in skeletal muscle mitochondrial dysfunction. In this study, we determined in skeletal muscle mitochondrial function in children with severe burns (n=8) at discharge from hospital and following 6 weeks of rehabilitative exercise (REX). Mitochondrial respiration was determined in permeabilized myofibers following the addition of substrates, ADP, succinate, and oligomycin. After which, the respiratory control ratio (RCR), substrate control ratio (SCR), and coupling control ratio (CCR) were calculated. Maximum mitochondrial respiration significantly increased after exercise training (12.7±2.1 pmol/mg/sec vs. 38.6±5.4 pmol/mg/sec, p<0.01), RCR (1.0 vs. 1.5) and SCR (1.1 vs. 1.7) increased while the CCR decreased (0.7 vs. 0.4) post REX. Skeletal muscle mitochondrial function is increased in burn victims following REX. Increased RCR and SCR indicate greater ADP and succinate sensitivity. The decrease in CCR suggests that mitochondria are more coupled post REX. These data suggest that early REX restores skeletal muscle mitochondrial function in burn survivors. Grant Funding Source : Supported by NIH (P50‐GM60338, R01‐HD049471, R01‐GM056687), and SHC (84090, 84080 and 84085) grants.