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Antioxidant activity of currently available resuscitation fluids in vitro (1157.5)
Author(s) -
Barr Johnny,
Jacobson David,
Grubbs Dana,
Dubick Michael
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1157.5
Subject(s) - antioxidant , resuscitation , chemistry , superoxide dismutase , hypertonic saline , albumin , tonicity , saline , pharmacology , biochemistry , medicine , anesthesia
Addition of antioxidants to resuscitation from hemorrhage reportedly improves outcomes in experimental animals. The present study investigated whether currently available resuscitation fluids have intrinsic antioxidant activity. Direct antioxidant activity of normal saline, hypertonic saline alone or with Dextran, Ringer’s solution alone or with lactate, pyruvate, or β‐hydroxybutyrate, Hextend, Voluven, plasma, 5% albumin and Hyvisc drag‐reducing compounds were investigated in duplicate in systems to either inhibit xanthine oxidase (XO) or superoxide dismutase (SOD) generated oxidant production or measure antioxidant status. N‐acetylcysteine (NAC) was included as a positive control. In the XO system, Hextend showed minor antioxidant activity, whereas Hyvisc preparations, plasma and Ringer’s showed antioxidant activity similar to NAC. All fluids tested inhibited superoxide production in the SOD system about 10% compared to over 30% by NAC. There did not appear to be any significant difference between colloids and crystalloids or compounds such as pyruvate or albumin at the concentrations selected. Taken together these data suggest that currently available resuscitation fluids have minor, if any, intrinsic antioxidant activity. Reported antioxidant properties of these compounds in vivo, most likely reflects indirect activity through their interactions with biological systems. Grant Funding Source : US Army Medical Research Materiel Command