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Increased type VI collagen gene expression in the post‐MI human myocardium (1152.11)
Author(s) -
Bryant Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1152.11
Subject(s) - myofibroblast , extracellular matrix , fibrosis , gene expression , myocardial infarction , cardiac function curve , medicine , ctgf , type i collagen , ventricular remodeling , heart failure , pathology , cardiology , gene , chemistry , growth factor , biochemistry , receptor
Cardiac fibroblasts and myofibroblasts are responsible for post‐myocardial infarction (MI) remodeling via regulation of extracellular matrix (ECM) formation. Accelerated post‐MI remodeling leads to excessive ECM deposition and fibrosis which can contribute to impaired contractile function, arrhythmias, and heart failure. Type VI collagen has been shown to induce myofibroblast differentiation in cultured cardiac fibroblasts and could contribute to accelerated post‐MI remodeling. Type VI collagen is elevated in the myocardium 7 days, 14 days, and 20 weeks post‐MI in the rat. The absence of type VI collagen improved cardiac function post‐MI in a knock out mouse model. The purpose of this study was to determine if collagen VI gene expression was elevated in human myocardium post‐MI. Autopsy samples were obtained from subjects who had died within seven days of an MI (acute MI group) and subjects who had died of a non‐heart related issue with no documented prior MI (control group). Quantitative real time PCR reactions were performed with RNaseP as a reference gene for normalization. Type VI collagen gene expression in the acute MI group was 414% greater than the control group expression, but did not reach statistical significance in this small study. However, this preliminary data indicate that type VI collagen gene expression may be significantly elevated in the post‐MI human myocardium and warrants further study. Grant Funding Source : Supported by Shenandoah University School of Pharmacy Professional Practice Plan