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Manganese accumulates in leaves of radishes grown in manganese supplemented soils (1143.10)
Author(s) -
ClarkeLambert Shellyann,
Stapleton Melissa,
Ruddock Karl,
Skeete Dereck
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1143.10
Subject(s) - manganese , soil water , fertilizer , chemistry , graphite furnace atomic absorption , environmental chemistry , trace element , horticulture , atomic absorption spectroscopy , biology , mass spectrometry , chromatography , physics , organic chemistry , quantum mechanics , ecology
Manganese (Mn) is a naturally occurring element, essential in trace amounts for living organisms, but is potentially toxic in high concentrations. Certain occupations including mining, welding and steel manufacturing can expose workers to chronically high levels of airborne Mn, leading to a clinical condition known as Manganism, which has Parkinson like symptoms. Recent studies report excess dietary Mn can impair immune and reproductive functions in birds. Previously we showed Mn is present in some commercially available fertilizers. We hypothesize plants grown in soils high in Mn or supplemented with fertilizers containing Mn will accumulate Mn in leaves and fruits. To test this we grew radishes in soils supplemented with fertilizers containing Mn, as well as in soil without added Mn. Samples (0.5 g) of each of the fertilizers as well as radish leaves were digested with HNO3 in a CEM Discovery Microwave Digester. Digested samples were analyzed for Mn levels using electrothermal vaporization with deuterium lamp background correction in a Perkin Elmer AA800 Atomic Absorption spectrophotometer with a THGA graphite furnace. We found that leaves from plants grown in soils supplemented with Mn accumulated Mn up to about 104 µg/gm tissue. The control leaves contained 25.28 µg/gm of Mn, which was significantly less than all the experimental groups. On the other hand, the soil that contained fertilizer had a significantly higher concentration of Mne than the plant tissue. The highest concentration of Mn recorded in the soil with Mn supplement fertilizer was 1709 µg/gm, while the lowest concentration was 1280 µg/gm. The study shows that plants will accumulate Mn from the soil and that use of fertilizers with high concentrations of Mn will increase the accumulations, possible creating a situation where animals and people ingesting the fruits and vegetables might be subjected to elevated Mn levels. Grant Funding Source : Supported by 0516041071 of NYSDOE and 0622197 of the DUE Program of NSF