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Cytotoxicity of peracetic acid in L929 fibroblasts (1139.2)
Author(s) -
Faria Gisele,
Viola Kennia,
Carlos Iracilda,
Kuga Milton,
GuerreiroTanomau Juliane,
TanomaruFilho Mario
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1139.2
Subject(s) - cytotoxicity , peracetic acid , sodium hypochlorite , chemistry , viability assay , root canal , mtt assay , neutral red , staining , cytotoxic t cell , biochemistry , microbiology and biotechnology , cell , in vitro , dentistry , biology , hydrogen peroxide , pathology , medicine , organic chemistry
Recently peracetic acid (PA) has been reported in the dental literature as a possible root canal irrigating solution. It has the advantage of bringing together in one product antibacterial activity and ability to remove smear layer. For selection of root canal irrigating solution it should take into account not only its therapeutic benefits, but also the possible cytotoxic effects, since it can contact the periapical tissues. The aim of this study was to evaluate the cytotoxicity of PA at different concentrations in comparison with 2.5% sodium hypochlorite (NaOCl) through the study of the L929 fibroblasts metabolism and the cytoskeleton. L929 fibroblasts in culture were exposed to different concentrations of PA and NaOCl and to the culture medium for 24 hours (control). After this period, it was evaluated the cell metabolism by methyl tetrazolium (MTT) assay and the cytoskeleton by staining actin and α‐tubulin. Data were analyzed by two‐way ANOVA and Bonferroni post test (α = 0.05). The solutions had an effect on cell viability/metabolism in a dose‐dependent manner. The cell viability decreased progressively as the concentration of irrigating solutions was increased. 0.5% AP showed similar cytotoxicity to 2.5% NaOCl (p>0.5); the AP at concentrations of 1%, 2% and 2.5% showed higher cytotoxicity than 2.5% NaOCl (p<0.5). The cytoskeleton suffered progressive structural changes as the concentrations of the solutions were increased. It was concluded that the AP is more cytotoxic than NaOCl. However, further studies evaluating other cell parameters and employing other PA‐based solutions need to be performed. Grant Funding Source : Supported by São Paulo Research Foundation (FAPESP) #2012/18203‐2