Premium
Prophylactic effects of melatonin with treadmill exercise on cartilage damage of rats with collagenase‐induced knee (1139.15)
Author(s) -
Hong Yunkyung,
Jin Yunho,
Choi Jeonghyn,
Hong Yonggeun
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1139.15
Subject(s) - melatonin , collagenase , cartilage , matrix metalloproteinase , medicine , chondrocyte , endocrinology , extracellular matrix , metalloproteinase , treadmill , anatomy , chemistry , enzyme , biochemistry
The purpose of this study is to evaluate effect of melatonin and exercise on extracellular matrix synthesis against cartilage damage in rats with collagenase‐induced knee laxity. Melatonin was injected twice/day subcutaneously, and treadmill exercise was conducted for 1‐hr/day. Intervention did not alter joint parameters, of which were significantly augmented in vehicle. Serum IL‐6 and TNF‐α was upregulatedin vehicle, which was attenuated by intervention. Melatonin combined with exercise decreased catabolic mediators but also cellular death with lower mineralization. TGF‐β1, which was reduced in vehicle, was augmented by intervention at early phase. At advanced phase, melatonin alone promoted mineralization in the cartilage as well as apoptosis, and melatonin blocked bone erosion and osteophyte formation. Combined intervention increased IL‐6, HIF‐2α, and MMP‐13 with COL2A1. Histological images exhibited rounded chondrocyte shape, smooth cartilage surface, and clear tidemark in combined intervention. Inhibition of subchondral bone erosion was detected in micro‐CT 3D image. Co‐treatment did exert non‐significance of trabecular parameters between both limbs. We suggest that melatonin with treadmill exercise may havepreventive and synergistic effect on cartilage degeneration, of which was more effective at initial phase.