Premium
Effect of Capsosiphon fulvescnes extract and pheophorbide A on glucose metabolism and oxidative stress in liver of streptozotocin‐induced diabetic rats (1139.10)
Author(s) -
Kim SeWook,
Oh Jisun,
Hong ChungOui,
Nam MiHyun,
Yang SungYong,
Jung HaYoung,
Lee KwangWon
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1139.10
Subject(s) - medicine , streptozotocin , endocrinology , chemistry , glutathione , oxidative stress , triglyceride , superoxide dismutase , malondialdehyde , antioxidant , alkaline phosphatase , diabetes mellitus , cholesterol , biochemistry , enzyme
We carried out to evaluate the effect of Capsosiphon fulvescnes extract (CFE) and Pheophorbide A (PhA) on blood glucose level and glucose tolerance. Male Sprague‐Dawley rats (160‐200 g), 9 weeks old, were administered with 40 mg/kg B.W. of streptozotocin (STZ) intraperitoneally to induce experimental diabetes. When we repeatedly administrated CFE and PhA for 9 weeks, administrations of CFE and PhA significantly improved glucose tolerance of STZ‐induced rats. Hyperglycemic effect of CFE was observed in an oral glucose tolerance test (OGTT) in which, a dose of CFE (4, 20 mg/kg B.W.) and PhA (0.2 mg/kg B.W.). An OGTT of rats treated low dose of CFE (CL), high dose of CFE (CH), and PhA decreased than it of STZ‐induced rats. Group treated CFE and PhA significantly reduced the serum glucose level, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total cholesterol and total triglyceride compared with the levels of STZ‐ induced rats group (diabetic control group). To confirm the hepatic protective effect, reduced glutathione (GSH), oxidized glutathione (GSSG), malondialdehyde (MDA) and antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) levels were measured in liver cytosol. Groups treated CFE and PhA increased the levels of GSH, SOD and CAT than that of STZ‐induced group significantly. And the levels of MDA and GSSG decreased compared with the levels of STZ‐induced group. Therefore, we suggested that PhA, activity compound of CFE, has hyperglycemic effects and ameliorate diabetic state.