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The effect of attenuating age‐related increases in blood pressure in male and female spontaneously hypertensive rats on the kidney (1136.17)
Author(s) -
Li Beverly,
Tipton Ashlee,
Sullivan Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1136.17
Subject(s) - endocrinology , medicine , blood pressure , kidney , excretion , hydrochlorothiazide
The kidney is critical in the long‐term control of blood pressure, and renal inflammation has been linked to the progression of hypertension. Previous experiments have shown a sex difference in protein excretion and the renal immune cell profile: male SHRs have greater indices of renal injury and more pro‐hypertensive renal T helper17 cells (Th17) while females had more BP‐lowering T regulatory cells (Tregs). Male and female SHR were randomized to receive tap water (vehicle) or the BP‐lowering drugs, hydrochlorothiazide and reserpine, in drinking water from 5 to 12 weeks of age. BP was measured by tail cuff, and kidneys were isolated for flow cytometric analysis of T cells. Treatment attenuated age‐related increases in BP in males (174±4 vs. 137±4 mmHg, p<0.05) and females (132±4 vs 161±1 mmHg, p<0.05). However, treatment did not significantly change metabolic parameters, urinary electrolyte excretion (Na+, K+, Cl‐) or urinary protein excretion in either sex. In contrast, although attenuation of the development of hypertension did not significantly change the renal T cell profile in male SHR, females exhibited a decrease in renal Tregs (p=0.0091) and Th17 (p=0.0203) counts which abolished the sex differences observed in the renal T cell profile of vehicle‐treated rats.