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Femoral artery occlusion amplifies TRPA1 function in skeletal muscle sensory neurons (1132.5)
Author(s) -
Xing Jihong,
Lu Jian,
Li Jianhua
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1132.5
Subject(s) - trpv1 , agonist , dorsal root ganglion , transient receptor potential channel , femoral artery , medicine , chemistry , nociception , neuroscience , sensory system , reflex , anesthesia , anatomy , receptor , dorsum , biology
A prior study showed that transient receptor potential channel A1 (TRPA1) in thin‐fiber muscle afferents contributes to the amplified reflex autonomic responses observed in rats with femoral artery ligation. Thus, in this study we characterized current responses induced by activation of TRPA1 in skeletal muscle dorsal root ganglion (DRG) neurons of control limbs and limbs with 24 hrs of femoral ligation using whole‐cell patch clamp methods. Our results show that AITC (a TRPA1 agonist, 50‐200 µM) produced a dose‐dependent increase of amplitudes of inward current responses. Also, the peak current amplitude induced by AITC is significantly larger in DRG neuron of ligated limbs. There is no difference in size distribution of DRG neurons with AITC‐induced current responses between two groups. However, arterial occlusion increases the percentage of the AITC‐sensitive DRG neurons compared with control. AITC‐induced currents in DRG neurons are significantly attenuated by exposure to 10 µM of HC‐030031, a selective inhibitor of TRPA1. In addition, capsaicin (a TRPV1 agonist) evokes a greater increase in the amplitude of AITC‐currents in DRG neurons of ligated limbs. Overall, our data suggest that a greater TRPA1 function is developed in muscle afferent nerves with the hindlimb ischemia and TRPV1 is partly responsible for augmented TRPA1 responses induced by arterial occlusion. Grant Funding Source : NIH P01 HL096570 and AHA EIA 0840130N