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Regulation of leptin receptor expressing neurons in the brainstem by TRPV1 (1126.11)
Author(s) -
Zsombok Andrea,
Jiang Yanyan,
Anwar Imran,
RezaiZadeh Kavon,
MuenzbergGruening Heike
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1126.11
Subject(s) - trpv1 , dorsal motor nucleus , neurotransmission , brainstem , neuroscience , leptin receptor , biology , transient receptor potential channel , vagus nerve , medicine , leptin , excitatory postsynaptic potential , premovement neuronal activity , receptor , endocrinology , inhibitory postsynaptic potential , stimulation , obesity
The central nervous system plays a critical role in the regulation of feeding and whole body metabolism via controlling the autonomic output to the visceral organs. Activity of the parasympathetic neurons in the dorsal motor nucleus of the vagus (DMV) determines the vagal tone and thereby modulates the function of the organs. Due to the increased prevalence of eating and metabolic disorders, there is a constant search for new compounds, receptors or modulators to alter the activity of autonomic output and thus influence feeding or metabolism. TRPV1 (transient receptor potential vanilloid type 1) plays a pivotal role in synaptic regulation and here, we tested the hypothesis that TRPV1 regulates the activity of leptin receptor (LepRb) expressing DMV neurons. First, whole‐cell patch‐clamp recordings were performed to determine the effect of TRPV1 activation on LepRb neurons. Capsaicin, a TRPV1 agonist increased the frequency of mEPSCs in 50% of the LepRb neurons. Next, we identified stomach‐projecting LepRb neurons in the DMV using the transsynaptic retrograde viral tracer, PRV‐614. Activation of TRPV1 increased excitatory neurotransmission to stomach‐related LepRb neurons. These data demonstrate that TRPV1 is involved in the regulation of a subpopulation of LepRb DMV neurons via presynaptic mechanism and suggest a potential interaction between TRPV1 and leptin signaling in the DMV. Grant Funding Source : NIH P30GM103337, Tulane Pilot Project