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NHE8 in human conjunctival epithelial cells: expression and regulation (1122.3)
Author(s) -
Zhao Yang,
Li Jing,
Wang Mingwu,
Ghishan Fayez,
Xu Hua
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1122.3
Subject(s) - inflammation , pathogenesis , tumor necrosis factor alpha , messenger rna , biology , immunology , microbiology and biotechnology , medicine , pathology , cancer research , gene , genetics
Dry eye disease (DED) is a disorder of the tear film by symptoms of dryness and irritation. Although the pathogenesis of dry eye is not fully understood, it is recognized that inflammation plays prominent role in the development and propagation of the debilitating condition. We have showed previously that loss of NHE8 function in mice resulted in dry eye syndrome. Therefore, we wanted to know if inflammatory cytokines (IL‐1β, IL‐6, IL‐17A and TNF‐α) which are detected in dry eye patients regulate NHE8 expression in the conjunctival tissue. Our results showed that NHE8 protein abundance was dramatically reduced in cells exposured to IL‐6, IL‐17A and TNF‐α in human conjunctival epithelial cells. The reduction on NHE8 protein expression was likely through inhibition of NHE8 mRNA expression in the case of IL‐1β, IL‐17A and TNF‐a. In summary, our study suggested that inflammatory cytokines regulate NHE8 expression at both protein and mRNA levels in dry eye condition. Grant Funding Source : Supported by NIH grant R01DK073638