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Butyrate increases and propionate decreases the frequency of propulsive contractions in guinea pig proximal colon (1110.7)
Author(s) -
Hurst Norm,
Kendig Derek,
Murthy Karnam,
Grider John
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1110.7
Subject(s) - propionate , butyrate , chemistry , distal colon , short chain fatty acid , guinea pig , medicine , fermentation , endocrinology , biochemistry , biology
Colonic bacteria produce short‐chain fatty acids (SCFAs) by fermentation of dietary carbohydrates and fiber. The production of SCFAs is greatest in proximal colon where propulsion is likely to be highly dependent on chemical/nutrient stimuli. AIM: To determine the effect of individual SCFAs on propulsive contractions in guinea pig proximal colon. METHODS: Proximal colon was excised, cannulated and placed in Kreb’s buffer in an organ bath. After equilibration, the colon was distended with 1 ml of Krebs’s buffer alone or containing sodium salts of acetate, butyrate, or propionate at 10‐100 mM. Motility was videorecorded, spatiotemporal maps generated, and the number of full‐length propulsive contractions during a 5 min period determined. RESULTS: The basal rate of propulsive contractions in Kreb’s buffer was 2.9±0.7 per 5 min. Butyrate and propionate had concentration‐dependent and opposing effects on propulsive contractions; acetate had no effect. Butyrate significantly increased propulsive contractions (maximal increase of 207% (p<0.05) at 30 mM) whereas propionate abolished propulsive contractions (91‐100% inhibition at 10‐30 mM). Control Kreb’s buffer containing 10‐100 mM NaCl had no effect. CONCLUSION: SCFAs have differential effects on propulsive contractions in guinea pig proximal colon with butyrate being excitatory and propionate being inhibitory. Grant Funding Source : Supported by DK34153