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Transcriptome‐wide RNA sequencing analysis of rat skeletal muscle feed arteries: impact of exercise training in obesity (1106.23)
Author(s) -
Padilla Jaume,
Jenkins Nathan,
Thorne Pamela,
Martin Jeffrey,
Rector R,
Davis J,
Laughlin M
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1106.23
Subject(s) - downregulation and upregulation , transcriptome , skeletal muscle , sprint , medicine , gene expression , endocrinology , gene , biology , physical therapy , genetics
We employed next generation RNA sequencing (RNA‐Seq) technology to determine the extent to which exercise alters global gene expression in skeletal muscle feed arteries and aortic endothelial cells (AEC) of obese OLETF rats. Transcriptional profiles of the soleus and gasctrocnemius muscle feed arteries (SFA and GFA, respectively) and AEC‐enriched samples from rats that underwent an endurance exercise training program(EndEx; n=12), interval sprint training program (IST; n=12), or remained sedentary (Sed; n=12) were examined. In response to EndEx, there were 39 upregulated and 20 downregulated genes in SFA and 1 upregulated and 1 downregulated gene in GFA (FDR < 10%). In response to IST, there were 305 upregulated and 324 downregulated genes in SFA and 101 upregulated and 66 downregulated genes in GFA, with an overlap of 32 genes between arteries. Furthermore, in AEC, there were 183 up‐regulated and 141 downregulated genes with EndEx and 71 upregulated and 69 downregulated genes with IST, with an overlap of 35 between exercise programs. Only expression of two genes (i.e., Tubb2b and Slc9a3r2) was altered (i.e., increased) by exercise in all three arteries. The finding that both EndEx and IST produced greater transcriptional changes in the SFA compared to the GFA is intriguing when considering the fact that treadmill exercise is associated with greater relative increases in blood flow to the gastrocnemius muscle compared to the soleus muscle. Grant Funding Source : NIH RO1HL036088