Premium
Radiation combined with hind‐limb suspension affects physiological parameters in rats (1103.7)
Author(s) -
Chowdhury Parimal,
Long Ashley,
Akel Nisreen,
Gaddy Dana,
Griffin Robert,
Bailey Lauren,
Dobretsov Maxim
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1103.7
Subject(s) - hindlimb , chemistry , tibia , muscle atrophy , soleus muscle , malondialdehyde , medicine , endocrinology , atrophy , anatomy , oxidative stress , skeletal muscle
This study examines acute and degenerative tissue responses to space‐like radiation doses in a rodent model of simulated microgravity. Three groups of rats, control (CON), irradiated (IR) and irradiated plus suspended (IR+HLS) were maintained for two weeks. IR and IR+HLS groups were exposed to radiation dose of 10GY. Body weights, soleus muscle weights, malondialdehyde (MDA), insulin/glucose levels, pressure‐pain threshold (PPT), grip force and heat pain were measured. CON gained weight faster than IR or IR+HLS. HLS but not IR resulted in soleus muscle atrophy. Bone mineral density in the proximal tibia was compromised by HLS and decreased by IR+HLS. Random plasma glucose levels were not affected by IR or IR+HLS. IR increased plasma insulin levels. PPT was increased in CON, remains unchanged in IR and decreased in (IR+HLS). MDA in plasma or in soleus muscle remain almost unchanged in CON, IR and (IR HLS) rats. However, IR significantly increased soleus muscle Cyt / Arg ratio over CON. Grip force, and/or low back pain did not differ between CON, IR and IR+HLS groups. No effects of irradiation were seen in heat pain threshold measurements. IR eliminated “hypoalgesia” and (IR+HLS) produced hyperalgesia. Moreover, IR exacerbated the HLS‐induced bone loss in the proximal tibia. These data suggest that radiation induces major physiological changes and these changes are exacerbated by exposure to HLS. Grant Funding Source : Arkansas Space Grant Consortium