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Radiation induced inhibition of myogenesis following muscle damage from cardiotoxin injection (1102.37)
Author(s) -
Cotter Joshua,
Baker Michael,
Acharya Munjal,
Limoli Charles,
Caiozzo Vincent
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1102.37
Subject(s) - myogenesis , cardiotoxin , regeneration (biology) , skeletal muscle , h&e stain , myocyte , andrology , medicine , tibialis anterior muscle , anatomy , chemistry , pathology , biology , staining , microbiology and biotechnology
Skeletal muscle is routinely exposed to radiation during diagnostic screening and therapeutic management of disease which may inhibit myogenesis. Previously it has been demonstrated in tissue culture that clinical doses of radiation blunt the activation of myogenic stem cells, referred to as “satellite” cells, which are important for the regeneration of skeletal muscle from injury/disease. The objective of the current study was to investigate the effects of radiation on myogenesis in the tibialis anterior (TA) following muscle damage from cardiotoxin (CTX). Adult male mice (C57BL/J6) were assigned to one of four groups: 1) CTX only ‐ 4 days, 2) CTX + irradiation (IRR) ‐ 4 days, 3) CTX only ‐ 14 days, or 4) CTX + IRR ‐ 14 days. Following injection, mice in the irradiation groups were exposed to a dose of 7 Gy of gamma irradiation; a clinically relevant dose of radiation. Following each time period, the TA was harvested and prepared for histological analysis by hematoxylin and eosin staining. The number of regenerative fibers was examined within the zone of damage. Regenerative fibers were identified by those fibers with centralized nuclei. CTX injection resulted in significant damage and subsequent fiber regeneration in all groups. Muscle regeneration was blunted in the CTX+IRR group as fibers with centralized nuclei were not observed until 14 days following CTX injection. Extensive regeneration was evident at 4 days and nearly complete at 14 days in the CTX group. The results indicate that a single, clinically relevant dose of radiation resulted in significant inhibition of myogenesis following muscle degeneration in an in vivo mouse model.