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Bone marrow inoculation of Walker 256 tumor cells in rats as a model of cancer cachexia (1102.36)
Author(s) -
Alves Christiano,
Almeida Ney,
Neves Willian,
Gutierrez Vanessa,
Voltarelli Vanessa,
Cury Yara,
Gualano Bruno,
Seelaender Marília,
Lancha Junior Antonio,
Brum Patricia
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1102.36
Subject(s) - bone marrow , medicine , atrophy , cachexia , treadmill , endocrinology , cancer
Bone marrow inoculation of Walker 256 tumor cells in rats as a model of cancer cachexia Christiano R. R. Alves, Ney R. de Almeida, Willian das Neves, Vanessa P. Gutierrez, Vanessa Voltarelli, Yara Cury, Bruno Gualano, Marília Seelaender, Antonio H. Lancha Junior, Patricia C. Brum. University of São Paulo, São Paulo, Brazil. The aim of this study was to standardize a model of cancer cachexia in rats by inoculating Walker 256 tumor cells in bone marrow. Male Wistar rats were inoculated with Walker 256 tumor cells in the bone marrow by an osteotomy in femur (Walker group). Control rats were submitted to sham osteotomy (Control group). Walker was compared to the Control group by unpaired Student's t ‐test. The significance level was previously set at p < 0.05. After the osteotomy, Walker group showed 16 days of survival on the average with 100% mortality rate after 23 days paralleled by lower body mass (‐8.8%; p = 0.03) and food intake (‐30.1%; p < 0.001) than Control group. In addition, Walker group decreased in vivo skeletal muscle function, assessed by both treadmill maximal incremental test (‐52.7%) and deambulation test (‐21.1%) (p < 0.001). Walker group displayed plantaris, gastrocnemius and triceps atrophy (p < 0.05 for all), which was not observed in soleus (p > 0.05). Additionally, lower citrate synthase activity and oxidized/reduced glutathione ratio were observed in plantaris (p < 0.05) but not in soleus (p > 0.05) of Walker group. These data provide evidence for oxidative metabolism dysfunction and redox imbalance in muscles with predominance of glycolytic fibers ( i.e. plantaris), but not in muscles with predominance of oxidative fibers ( i.e. soleus). Collectively, our results suggest that bone marrow inoculation of Walker 256 tumor cells in rats is a new model of severe cancer‐induced muscle atrophy selective to glycolytic fibers. Grant Funding Source : FAPESP (process: 2012/02528‐0)