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Temporal variation in skeletal muscle atrophic pathways following transection of the sciatic nerve (1102.31)
Author(s) -
Callahan Zachary,
Menke Chelsea,
Schaeffer Paul
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1102.31
Subject(s) - sciatic nerve , muscle atrophy , hindlimb , atrophy , medicine , western blot , skeletal muscle , sham surgery , anatomy , gene expression , surgery , endocrinology , anesthesia , pathology , chemistry , gene , biochemistry , alternative medicine
The purpose of this study was to investigate the temporal changes in gene expression and morphology in muscles of the hindlimb following transection of the sciatic nerve. A sham surgery was performed on the contralateral limb and was used as a control. Mice were sacrificed 3, 10, 28, or 56 days after surgery. There was no significant difference in muscle mass between sham and transected limbs at 3 days post‐surgery, but tissue mass was significantly lower following transection at the other three time points. Transected muscle mass stabilized by 28 days post‐surgery with no further loss. Myocellular cross sectional area was significantly smaller at 28 and 56 days post‐transection surgery. Additionally, tissue fibrosis area was significantly greater at 56 days post‐transection. In transected muscle, gene expression analysis revealed higher expression of MAFbx and MuRF‐1 (genes involved in atrophic pathways) at 3 days post‐surgery while expression was significantly lower at 28 days post‐surgery. We saw no difference in gene expression of Fox01 or Fox03 (two upstream regulators of MAFbx and MuRF‐1) for any of the conditions. Western blot analysis revealed that MuRF‐1 protein expression was significantly higher in transected muscle at all times except 56 days post‐surgery. Fox01 protein expression in transected tissues was significantly lower in 10, 28, and 56‐day groups. These data suggest that the transection of the sciatic nerve results in the activation of pathways that initially increase atrophy, but that long‐term effects are less clear. *This work was supported by NIH grant number 1R15DK085 497‐01A1. Grant Funding Source : Supported by NIH grant number 1R15DK085 497‐01A1