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The effects of doxorubicin administration on intramuscular nitric oxide concentration in rat skeletal muscle (1102.28)
Author(s) -
Lalanne Josee,
Fabris Sergio,
MacLean David
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1102.28
Subject(s) - skeletal muscle , nitric oxide , doxorubicin , chemistry , intramuscular injection , medicine , endocrinology , chemotherapy
Doxorubicin (DOX) is a frontline anticancer therapeutic which has proven effective in the treatment of breast cancer. However, very little is known regarding the effects that DOX has on skeletal muscle metabolism, specifically its influence on nitric oxide (NO) production. Therefore, the purpose of the present study was to determine NO concentrations in the plantaris (P, n =6) and gastrocnemius (G, n =6) muscles of Sprague‐Dawley rats after DOX administration at a single dose of 1.5 mg/kg. Muscle samples were collected at 24, 48, 72, 96, 120, 144, 168 and 192 hours post injection. . Following administration, intramuscular NO increased (P<0.05) in the G after 120 (0.99±0.04 mmol/kg dw), 144 (0.91±0.03 mmol/kg dw) and 168 hours (0.79±0.03 mmol/kg dw) compared to control (0.62±0.07 mmol/kg dw). Similarly, NO increased (P<0.05) in the P after 120 (0.89±0.02 mmol/kg dw) and 144 hours (0.89±0.03 mmol/kg dw) compared to control (0.66±0.08 mmol/kg dw). Other than the observation that NO remained higher in the G but not in the P group at 168 hr, there were no other differences between fiber types. These data clearly suggest that DOX administration stimulates intramuscular NO production in a time dependent manner. In addition, these findings may represent the first step in better understanding NO deregulation in skeletal muscle upon DOX administration. Grant Funding Source : Supported by NSERC